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KMID : 0903519970400060556
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1997 Volume.40 No. 6 p.556 ~ p.560
Production of ¥ä-Aminolevulinic Acid in Soybean Curd Wastewater by Rhodobacter capsulatus KK-10




Abstract
The removal efficiency of COD and the production of ¥ä-aminolevulinic acid (ALA) were concurrently investigated for both purifying the soybean curd wastewater of high BOD and utilizing the wastewater as a renewable substrate of ALA production using Rhodobacter capsulatus KK-10. Its wastewater was a favorable media for the growth of photosynthetic bacteria in terms of its environmental characteristics having COD/BOD rate of 0.98, ratio of BOD : N : P =100 : 6 : 4, BOD/N ratio of 17.2, lactic acid of 1,080 ppm. Its COD value wastewater was decreased to 94% and dry cell weight was approached to about 1.2 g/§¤ after cultivation of the photosynthetic bacteria for 4 days. By the addition of 15 mM levulinic acid (LA) into the wastewater at the middle log phase of cell growth, the amount of ALA secreted was 55 §·/§¤. The ALA production was considerably increased to 114 §·/§¤ under the cultural condition of 15 mM supplementations of glycine and succinate with LA at the same period. Furthermore the maximum ALA production of 120 §·/§¤ and COD removal efficiency of 92% were accomplished in the soybean curd wastewater enriched with one addition of 15 mM LA and three serial additions 15 mM ALA precursors.
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